Type of Document Master's Thesis Author LIU, YIDAN Author's Email Address yliu36@student.gsu.edu URN etd-04212009-133116 Title A NOVEL METHOD FOR THE QUANTITATIVE EVALUATION OF FIBRINOGEN COAGULATION Degree Master of Science Department Chemistry Advisory Committee
Advisor Name Title Binghe Wang Committee Chair Gangli Wang Committee Member Zhen Huang Committee Member Keywords
- Platelet
- Coagulation
- Fibrinogen
- APMSF
- PMSF
- Photograph
- AEBSF
- Thrombin inhibitor
- SELEX
- Aptamer
- Thrombin
Date of Defense 2009-04-16 Availability unrestricted Abstract Fibrinogen aggregation is the last step in blood coagulation. Inhibition of fibrinogen aggregation could lead to anticoagulation effects. However, there is no good method for the ready evaluation of fibrinogen coagulation. A commonly used path method is slow and requires an expensive instrument. In this project, we have developed a microplate reader and In evaluating inhibitors of fibrinogen coagulations there is no good method. As an important process in hemostasis, fibrinogen coagulation is often detected by micro-plate reader. In our test of fibrinogen coagulation, we improved the observing and analyzing method by using photograph to see concentration-depended effect of thrombin inhibitors on the coagulation. Three known thrombin inhibitors, AEBSF, APMSF and PMSF, were applied to develop the method for detecting the fibrinogen coagulation. The results showed our method is of accuracy in determination of the amount of fibrin when compared with other types of methods.Files
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