Type of Document Master's Thesis Author Seiscio, Andrew R URN etd-05142008-223402 Title The Role of GABAergic Transmission in Mediation of Striatal Local Field Potentials (LFPs) Degree Master of Science Department Neuroscience Advisory Committee
Advisor Name Title Dr. Andrew Clancy Committee Chair Dr. Thomas Wichmann Committee Co-Chair Dr. Kyle Frantz Committee Member Keywords
- Rhesus macaque
- synchronized oscillatory discharge
- gabazine
- microdialysis-local field potential probe
- local field potentials
Date of Defense 2006-04-14 Availability unrestricted Abstract In the present study, electrophysiological and behavioral effects of compromised Gama-Aminobutyric Acid (GABAergic) transmission were investigated in adult Rhesus macaque monkeys (N=2). GABAergic transmission was perturbed in the putamen by administration of a GABAa receptor antagonist, gabazine (10 and 500 μM), via a microdialysis-local field potential (MD-LFP) probe. Resultant changes in striatal local field potentials (LFPs) were measured as an assay of synchrony.
Gabazine perfusion evoked discrete large amplitude spikes in LFPs in all subjects, and the frequency and shape of individual spikes were concentration-dependent. Pre-treatment with the GABAa receptor agonist, muscimol (100 μM) blocked the gabazine-induced events, confirming a role for GABAa receptors in the effects. Behavioral manifestations of gabazine treatment were observed only at the maximum concentration. Unusual facial movements suggested aberrant electrical activity was propagated from striatum to motor cortex, perhaps via reentrant circuits. These results support a role for GABAergic transmission in segregation of striatal circuits.
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