Type of Document Master's Thesis Author Filfili, Chadi N URN etd-07242008-085158 Title PRODUCTION OF B VIRUS GLYCOPROTEIN D AND EVALUATION OF ITS DIAGNOSTIC POTENTIAL Degree Master of Science Department Biology Advisory Committee
Advisor Name Title Julia Hilliard, Ph.D. Committee Chair Barbara Baumstark, Ph.D. Committee Member Roberta Attanasio, Ph.D. Committee Member Keywords
- cercopithecine
- Serodiagnosis
- Herpes virus
- Glycoprotein D
- Recombinant
- ELISA
- Rhesus macaque
Date of Defense 2008-07-01 Availability unrestricted Abstract B virus diagnosis presents a challenge largely complicated by the asymptomatic infectionof rhesus macaques, and extremely pathogenic fatal infections in humans. Humoral
detection of antibodies is generally performed using whole virus antigen for which
preparation requires strict biosafety measures and specialized BSL-4 facilities. As an
alternative to utilizing B virus antigen, we describe the production of a truncated form of
B virus envelope glycoprotein D, gD 287, in a baculovirus expression system, and
evaluate its diagnostic potential as an antigen in recombinant ELISA. After purification
and characterization, gD 287 was tested using 22 negative and 72 positive macaque sera
samples previously classified using the traditional method. We find that sensitivity and
specificity of the recombinant ELISA are dependent on antibody titer of tested serum and
gD 287 shows good to excellent predictive potential for identification of positive sera
with titers higher than 500.
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