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Title page for ETD etd-07282006-120138


Type of Document Master's Thesis
Author Maxwell, Kerry Elizabeth
URN etd-07282006-120138
Title Neurolipofuscin is a measure of age in the Caribbean spiny lobster, Panulirus argus, in Florida
Degree Master of Science
Department Neurobiology & Behavior
Advisory Committee
Advisor Name Title
Charles Derby Committee Chair
Matthew Grober Committee Member
Rodney Bertelsen Committee Member
Thomas Matthews Committee Member
Keywords
  • Panulirus argus
  • Neurolipofuscin
  • Spiny Lobster
  • Florida Keys
  • Growth
Date of Defense 2005-11-30
Availability unrestricted
Abstract
Accurate age estimates for the commercially-important Caribbean spiny lobster, Panulirus argus, would greatly enhance analyses of life history and population dynamics. Previous estimates of their age based on size and growth may be inaccurate because of variable growth in the wild. An established technique for aging crustaceans – histologically-determined lipofuscin content in the nervous system – was used on lobsters reared in the laboratory for up to five years. We verified the presence of lipofuscin in eyestalk neural tissue and described its distribution in cell cluster A of the hemiellipsoid body. Neurolipofuscin content of both sexes increased linearly over the five-year age range, with seasonal oscillations. Growth of these animals, on the other hand, showed sex differences and began to asymptote after three years. Neurolipofuscin concentrations in the two eyestalks from the same animal were similar. These results suggest that the neurolipofuscin technique will be valuable for estimating age of wild-caught P.argus.
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