Type of Document Master's Thesis Author Johnson, Julian A Author's Email Address juju10683@yahoo.com URN etd-08252007-194959 Title THE PRODUCTION OF DESIGNED POTENTIAL PROTEIN CONTRAST AGENTS AND THEIR ENCAPSULATION IN ALBUMIN MICROSPHERES Degree Master of Science Department Biology Advisory Committee
Advisor Name Title Jenny J Yang Committee Co-Chair Zhi Ren Liu Committee Co-Chair Giovanni Gadda Committee Member Keywords
- Contrast agent
- Protein design
- CD2
- Microspheres
Date of Defense 2007-07-03 Availability unrestricted Abstract Using protein design, a series of metal binding proteins have been designed,allowing the local factors that contribute to metal affinity and thermostability to be
studied. Those proteins with the highest metal binding affinities had the lowest apo-form
Tm and the largest ÄTm upon metal binding. In this thesis, major steps have been taken
toward applying the engineered protein to MR imaging. The progress of magnetic
resonance imaging is hindered by low specificity and rapid elimination of FDA-approved
MRI contrast agents. The engineered protein contrast agent has been conjugated to a
cancer-specific targeting peptide and encapsulated in albumin microspheres to provide
tandem passive and active tumor targeting. Also, a simple, high-yield purification
method has been developed.
Files
Filename Size Approximate Download Time (Hours:Minutes:Seconds)
28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access JOHNSON_JULIAN_200712_MS.pdf 4.62 Mb 00:21:22 00:10:59 00:09:37 00:04:48 00:00:24